The Italian railway system, run by Trenitalia, is connected with the rest of the European rail system so it is easy to reach Tuscany from locations across Europe and Italy.
Overnight hauls give you the possibility to book a couchette (cuccetta) on most international trains. It is always advisable, and sometimes required, to book seats on international trains to and from Italy.
The main route that passes through Tuscany is through Florence, on the Bologna-Rome line via Arezzo. Another line from Rome hugs the Tyrrhenian sea coast and passes through Grosseto, Livorno, Pisa, Viareggio and on along the coast on its way to La Spezia and Genoa (in Liguria).